ABSTRACT
To determine the prevalence rates and serovar distribution of Chlamydia trachomatis cervical infections in Cuban women, two different groups were selected. Group I consisted of 60 human immunodeficiency virus (HIV-1) seropositive women from different regions of Cuba and group II of 60 randomly selected women HIV seronegative and apparently healthy. C. trachomatis was detected in cervical scrapes by mean of nested polymerase chain reaction (PCR) specific for major out membrane protein. The overall prevalence rate of C. trachomatis in cervical scrapes determined by nested PCR was 10 percent in group I and the estimated prevalence was 6.6 percent for group II; 83.3 percent of HIV seropositive women with C. trachomatis infection reported history of pelvic inflammatory disease followed by cervicitis (50 percent). The control group C. trachomatis-infected women referred a history of cervicitis in 75 percent of cases. Other reports in the latter group included infertility and pelvic inflamatory disease in 50 percent. The present study is the first report of C. trachomatis prevalence in Cuba. It showed that there was not significantly difference in the prevalence rate of C. trachomatis between both groups
Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Chlamydia Infections , Chlamydia trachomatis , HIV Infections , HIV-1 , Case-Control Studies , Chi-Square Distribution , Chlamydia Infections , Cuba , Parity , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Porins , Prevalence , Pelvic Inflammatory Disease/complications , Pelvic Inflammatory Disease/diagnosis , Pelvic Inflammatory Disease/epidemiology , Risk Factors , Uterine Cervicitis , Vaginal SmearsABSTRACT
Se describen los resultados del estudio de Enterovirus como agentes productores de meningoencefalitis viral, desde 1990 hasta 1994. Fueron estudiados en este periodo 546 muestras de heces fecales, 95 liquidos cefalorraquideos y 1 058 sueros pareados, obtenidos de 1 388 pacientes diagnosticados clinicamente con esta enfermedad. Las muestras para aislamiento viral se inocularon en 2 sistemas celulares diferentes, el mayor numero de aislamiento se encontro en celulas diploides de fibroblasto humano. Las determinaciones de anticuerpos se realizaron por prueba de neutralizacion (micrometodo) con 11 antigenos de Enterovirus (Echo 4,6,9,11 y 30 y Coxsackie B1,2,3,4,5 y 6) y en periodos epidemicos con el virus aislado. En los anos estudiados se produjeron 2 brotes epidemicos; uno por Coxsackie A9 (1990-1991) y otro por Echo 30 (1994). En los sueros pareados se encontro mayor positividad a los Echo 6 y 11